Properties of Proteus mirabilis and Providence spheroplasts.

نویسنده

  • A J Van Rensburg
چکیده

Proteus mirabilis strain I 3 and Providence strain NCTC 92 I I were converted to osmotically sensitive spheroplasts by growth in the presence of penicillin or glycine in sucrose-supplemented or unsupplemented medium. Lysazyme in the presence of tris and ethylenediaminetetra-acetic acid (EDTA) converted both these strains to spheroplasts. Penicillin spheroplasts could be stabilized with sucrose, streptomycin, spermine or di-valent cations. Electron micrographs of these spheroplasts demonstrated that the outer layer of the bacterial cell wall was retained. Penicillin-induced spheroplasts possessed receptor sites for bacteriophages.

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عنوان ژورنال:
  • Journal of general microbiology

دوره 56 2  شماره 

صفحات  -

تاریخ انتشار 1969